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SR-B1 expression is elevated in AML cells, targeted by bioinspired gold-core HDL NPs. (A) HDL NPs comprise a 5 nm gold NP core surface functionalized with ApoA-I and phospholipids. After the stepwise addition of the components and self-assembly, HDL NPs are purified by <t>tangential</t> flow <t>filtration.</t> (B) In the LAML database in Gene Expression Profiling Interactive Analysis, there was a significant reduction in OS with patients expressing higher SR-B1 levels than those with AML with lower SR-B1 expression. (C) SR-B1 expression by flow cytometry in AML cell lines MV4-11, HEL, U937 compared with SR-B1 – U266B1 and SR-B1 + Ramos cells presented as a histogram (left) and MFI (right). ApoA-I, apolipoprotein A1; HR, hazard ratio.
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SR-B1 expression is elevated in AML cells, targeted by bioinspired gold-core HDL NPs. (A) HDL NPs comprise a 5 nm gold NP core surface functionalized with ApoA-I and phospholipids. After the stepwise addition of the components and self-assembly, HDL NPs are purified by tangential flow filtration. (B) In the LAML database in Gene Expression Profiling Interactive Analysis, there was a significant reduction in OS with patients expressing higher SR-B1 levels than those with AML with lower SR-B1 expression. (C) SR-B1 expression by flow cytometry in AML cell lines MV4-11, HEL, U937 compared with SR-B1 – U266B1 and SR-B1 + Ramos cells presented as a histogram (left) and MFI (right). ApoA-I, apolipoprotein A1; HR, hazard ratio.

Journal: Blood Neoplasia

Article Title: Targeting scavenger receptor class B type 1 with a bioinspired ligand induces apoptosis or ferroptosis in AML

doi: 10.1016/j.bneo.2025.100122

Figure Lengend Snippet: SR-B1 expression is elevated in AML cells, targeted by bioinspired gold-core HDL NPs. (A) HDL NPs comprise a 5 nm gold NP core surface functionalized with ApoA-I and phospholipids. After the stepwise addition of the components and self-assembly, HDL NPs are purified by tangential flow filtration. (B) In the LAML database in Gene Expression Profiling Interactive Analysis, there was a significant reduction in OS with patients expressing higher SR-B1 levels than those with AML with lower SR-B1 expression. (C) SR-B1 expression by flow cytometry in AML cell lines MV4-11, HEL, U937 compared with SR-B1 – U266B1 and SR-B1 + Ramos cells presented as a histogram (left) and MFI (right). ApoA-I, apolipoprotein A1; HR, hazard ratio.

Article Snippet: HDL NPs were purified using the KrosFlo tangential flow filtration system, using a 50 kDa cutoff polyethersulfone module (Repligen).

Techniques: Expressing, Purification, Filtration, Gene Expression, Flow Cytometry